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CALSCALE:GREGORIAN
PRODID:UW-Madison-Physics-Events
BEGIN:VEVENT
SEQUENCE:5
UID:UW-Physics-Event-4873
DTSTART:20190201T213000Z
DURATION:PT1H0M0S
DTSTAMP:20260317T090846Z
LAST-MODIFIED:20190123T205711Z
LOCATION:2241 chamberlin Hall
SUMMARY:Making Movies of Molecules - The Application of X-ray Lasers \
 , Physics Department Colloquium\, Junko Yano\, LBNL
DESCRIPTION:Our group is studying the mechanisms of the catalytic reac
 tions in both natural and inorganic systems.  Using various X-ray tech
 niques as tools\, we are studying how the catalysts do multielectron r
 eactions\, by following the reaction under functional conditions.<br>

 \nWe have developed spectroscopy and diffraction techniques necessary 
 to fully utilize the capability of the X-ray Free Electron Laser facil
 ities (XFELs) for a wide variety of metalloenzymes\, and to study thei
 r chemistry under functional conditions. One of such methods is simult
 aneous data collection for X-ray crystallography and X-ray spectroscop
 y\, to look at the overall structural changes of proteins and the chem
 ical changes at metal catalytic sites. In parallel to the detection te
 chniques\, we have developed an efficient sample delivery method that 
 involves deposition of droplets on a conveyor belt. This 'Droplet on T
 ape' (DOT) method\, delivers a single drop of the crystal suspension o
 r solution sample onto a tape\, which then can be transported to the X
  ray intersection point with a variable delay in time. In the process\
 , the sample is photochemically or chemically activated at various tim
 e delays to capture reaction intermediates with crystallography and sp
 ectroscopy. <br>
\nThe presentation will cover our group's recent resu
 lts of metalloenzyme research\, in particular\, for the study of the w
 ater oxidation reaction in natural photosynthesis\, using X-ray crysta
 llography and X-ray spectroscopy at LCLS (LINAC Coherent Light Source)
 . 
\n<br>
\n<br>
\nReferences: <br>
\nKern\, J. et al. Simultaneous fe
 mtosecond X-ray spectroscopy and diffraction of
\nFuller\, F. D. et al
 . Drop-on-demand sample delivery for studying biocatalysts in action
\
 nat XFELs Nat. Methods 14\, 443 (2017). <br>
\nKubin\, M. et al. Soft 
 x-ray absorption spectroscopy of metalloproteins and high-valent metal
 -complexes at room temperature using free-electron lasers\, Struct. Dy
 namics 4\, 054307 (2017)<br>
\nKern\, J. et al. Structures of the inte
 rmediates of Kok’s photosynthetic water oxidation clock. Nature 563\
 , 421 (2018).
\n              
URL:https://www.physics.wisc.edu/events/?id=4873
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